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Table 25 Validation parameters for Vitamin C

From: Development and validation of a stability indicating green analytical method for the simultaneous estimation of l-glutathione, n-acetyl l-cysteine and Vitamin C in marketed formulation using UV–visible spectroscopy

Validation parameter

Vitamin C

Absorption maxima (nm)

264.5 nm

Linearity range

2–10 µg/ mL

Coefficient of regression (R2)

0.999

Regression equation

0.0547x − 0.0139

Slope (b)

0.0547

Intercept (a)

0.0139

Limit of detection (LOD)

0.18 µg/mL

Limit of quantification (LOQ)

0.57 µg/mL