Table 2 Analysis of variances (ANOVA) for study of significant main effect, two-way interactions and quadratic effects of critical procedure variables using central composite design and Minitab 18 software
Source | Degree of freedom (DF) | Adjusted sum of square | Adjusted mean square | F-Value | P-Value |
|---|---|---|---|---|---|
ANOVA for Response R1—Fluorescence intensity of PGB | |||||
Model | 5 | 23,123.5 | 4624.7 | 41.66 | 0.000 |
Linear | 2 | 17,141.6 | 8570.8 | 77.21 | 0.000 |
CPV—A | 1 | 13,654.0 | 13,654.0 | 123.01 | 0.000 |
CPV—B | 1 | 3487.6 | 3487.6 | 31.42 | 0.001 |
Square | 2 | 5956.7 | 2978.3 | 26.83 | 0.001 |
CPV—A*CPV—A | 1 | 3354.6 | 3354.6 | 30.22 | 0.001 |
CPV—B*CPV—B | 1 | 3379.1 | 3379.1 | 30.44 | 0.001 |
Two-way interaction | 1 | 25.3 | 25.3 | 0.23 | 0.648 |
CPV—A*CPV—B | 1 | 25.3 | 25.3 | 0.23 | 0.648 |
ANOVA for Response R2—%recovery of PGB | |||||
Model | 5 | 681.653 | 136.331 | 49.17 | 0.000 |
Linear | 2 | 502.583 | 251.292 | 90.64 | 0.000 |
CPV—A | 1 | 396.632 | 396.632 | 143.07 | 0.000 |
CPV—B | 1 | 105.951 | 105.951 | 38.22 | 0.000 |
Square | 2 | 178.381 | 89.190 | 32.17 | 0.000 |
CPV—A*CPV—A | 1 | 107.538 | 107.538 | 38.79 | 0.000 |
CPV—B*CPV—B | 1 | 94.042 | 94.042 | 33.92 | 0.001 |
Two-way interaction | 1 | 0.689 | 0.689 | 0.25 | 0.633 |
CPV—A*CPV—B | 1 | 0.689 | 0.689 | 0.25 | 0.633 |