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Future Journal of Pharmaceutical Sciences
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Design and evaluation of antifungal vaginal suppository using coconut oil as base for vulvovaginal candidiasis

Future Journal of Pharmaceutical Sciences volume 9, Article number: 80 (2023) Cite this article

Abstract

Background

The emergence of antimicrobial resistance to antifungals has made vulvovaginal candidiasis a concern. Coconut oil has antimycotic properties that could have a synergistic effect when combined with antifungals. Thus, clotrimazole suppositories were prepared using coconut oil as a base to improve its use and delivery in antimycotic treatment and the suppositories were evaluated for their physicochemical, mechanical, and drug release properties, and the antimycotic effect of clotrimazole and coconut oil was examined singly and in combination, as well as their formulations using the agar-well diffusion method in comparison with polyethylene glycol (PEG), and cocoa butter bases. Using the fusion method, coconut oil was solidified with beeswax (20–50%) to prepare 100 mg clotrimazole suppositories. Surfactants (4% w/w); Tween 20®, Span 20®, sodium lauryl sulphate, and their combinations (3% w/w ratio 1:1 of Tween 20® and Span 20®) were used to improve the rate of drug release from the suppository.

Results

The suppositories had a pH of 4.1–6.0 and crushing strengths of 0.53 ± 0.07–32.56 ± 5.42 N. Suppositories containing surfactants and those prepared from PEG had significantly (p < 0.05) lower disintegration times ranging from 35 to 90 min than those without surfactants ranging from 305 to 388 min. Drug release (t80) was the fastest from the suppositories containing 40% coconut oil, Tween 20, and PEG. Using the Korsmeyer–Peppas’ model, suppositories made from PEG had a non-Fickian diffusion, while those containing 40% coconut oil, and Tween 20 had a super case II transport mechanism. The combination of clotrimazole and coconut oil gave higher zones of inhibition against Candida species compared to either clotrimazole or coconut oil alone. The formulations had higher antimycotic activities against Candida albicans than Candida krusei and Candida tropicalis.

Conclusion

The optimized formulation with the desired physicochemical and drug release properties was obtained with coconut oil (40% w/w) solidified with beeswax (50% w/w) as a base. Coconut oil appeared to possess a synergistic antimycotic effect on clotrimazole. Thus, clotrimazole vaginal suppositories with coconut oil as a base in the treatment of vulvovaginal candidiasis showed potential against C. albicans and other Candida species.

Graphical abstract

Background

Vulvovaginal Candidiasis (VVC) is an opportunistic fungal infection, that in most cases is caused by Candida species with Candida albicans accounting for 85–95% [1, 2]. The five most associated species with vaginal candidiasis are C. albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis, and Candida krusei [3]. The pathological hallmark of the disease is an acute inflammatory condition of the vulva and vaginal mucosa induced and accompanied by the overgrowth of Candida organisms that normally exist in the vaginal mucosa as quiescent symbiotes, forming a complex vaginal ecosystem with other bacteria [4,5,6]. The disease is characterized by vulvar erythema, excoriation, pruritus, and an abnormal vaginal discharge, which can be “cheese-like” or watery though it has been estimated that about 33% of all women exhibit no symptoms [2, 7]. An estimated 138 million women globally experience recurrent VVC annually with associated morbidity of pain, altered self-esteem, poor work performance, discomfort, interference with sexual and affective relations, and mental distress [2, 8,9,10]. The main predisposing factor to VVC is the use of antimicrobial medications that destroy beneficial Lactobacillus bacteria of normal vaginal microbiota while other factors are; uncontrolled diabetes mellitus, immunosuppressive conditions, genetic predispositions, the use of glucocorticoids, oral contraceptives, or hormone replacement therapy, pregnancy, sexual activities and tight-fit clothing [4, 8, 11,12,13]. According to historical data, the prevalence of VVC is higher in the age group of 25–34 years, with an estimated 70% of all women experiencing at least one episode throughout their reproductive years [4, 7, 10, 13, 14].

The vaginal route for drug administration has many potential benefits because it has a large surface area for drug absorption, relatively low enzymatic activity, avoids first-pass effects, and has ease of administration [15]. Like topical Candida infections, vulvovaginal Candidiasis is treated effectively with azole-based antifungal drugs. However, such therapy can be complicated by the emergence of drug-resistant yeasts [16, 17]. This emergence of antimicrobial resistance to antifungals has made vulvovaginal candidiasis a concern [18]. The issue of drug-resistant Candida species may have been exacerbated by using over-the-counter (OTC) products for self-treatment [16, 17]. Most of the OTC drugs are azole-based, thus, the frequent or prolonged use of these products may have the potential for widespread drug resistance [19].

The composition of the suppository base is an important factor in the release process [20]. Suppository bases have been classified into oleaginous, aqueous, and emulsifying bases. The oleaginous bases are known for their ease of formulation, non-irritating, and non-sensitizing nature, though they will require refrigeration in the tropics due to their melting point ranges [21].

Coconut oil (CO), is an edible oil obtained from Cocos nucifera Linn, family Arecaceae [22] with saponification and iodine values of 248–268 mgKOH/g and 4.1–11.0, respectively [23, 24]. Coconut oil has been reported to have antimicrobial properties against gram-positive and gram-negative bacteria, fungi, protozoa, and viruses [25] due to the presence of medium-chain fatty acids—lauric, caprylic and capric acids [26]. It has been suggested that the mechanism for the antimycotic effect of coconut oil is that the membrane lipids of the fungi are solubilized as the fatty acids integrate into the membrane. The antimycotic effect of the coconut oil alone, however, is not adequate to treat VVC. Thus, combined with antifungals, coconut oil’s antimycotic properties could be synergised [27].

Clotrimazole an azole antifungal drug belonging to the imidazole group is well-tolerated with broad-spectrum antimycotic activity majorly used for the treatment of C. albicans and other fungal infections [28]. It acts by inhibiting the synthesis of the fungal membrane's sterol components, thereby impairing ergosterol synthesis and leading to a cascade of membrane abnormalities in the fungus [29].

In the present study, clotrimazole suppositories were designed for the immediate release of clotrimazole using coconut oil as a base to improve their use in antimycotic treatment in comparison with polyethylene glycol and cocoa butter bases.

Materials and methods

Materials

Virgin coconut oil (obtained from a market in Akobo, Ibadan, Nigeria). Beeswax, cocoa butter (purchased from Showcrown Laboratory Technologists, Ibadan, Nigeria), Tween 20®, and Span 20® (BDH Chemical Ltd. Poole, England), Sodium Lauryl Sulphate, polyethylene glycol (MW 8000) (Loba Chemie Pvt. Ltd. Mumbai, India), Sabouraud dextrose agar (LAB M Ltd, Lancashire, UK) and Clotrimazole (a gift from Bond Chemical Industries Limited Awe, Oyo State, Nigeria). Candida albican was obtained from the Medical Microbiology Laboratory, University College Hospital Ibadan, while C. tropicalis and C. krusei were obtained from the Pharmaceutical Microbiology Laboratory, University of Ibadan, Nigeria.

Methods

Preparation of suppository

Coconut oil was solidified with different concentrations (20, 30, 40 and 50%) of beeswax to formulate 100 mg clotrimazole suppositories using the fusion method [21]. The displacement value of the suppository base was determined using a 1 g mould. Batches of suppositories made from cocoa butter, polyethylene glycol, and those containing 4% w/w Tween 20®, Span 20®, sodium lauryl sulphate and a combination of 3% w/w Tween 20® + Span 20®, were prepared by adding them into the modified base before the addition of clotrimazole. The molten preparation was carefully poured into pre-calibrated stainless-steel moulds and cooled in the refrigerator to solidify at 4 °C. It was then stored at room temperature (30 ± 2 °C) for 24 h after removal from the mould to allow for uniform solidification and crystal transformation [21]. The suppositories were then stored in a desiccator wrapped in aluminium foil in a refrigerator until needed. The formulation composition as well as the formulation codes are shown in Table 1.

Table 1 The formula of vaginal suppositories
Full size table

Evaluation of clotrimazole suppositories

pH

The pH of the molten suppositories was determined using a calibrated pH meter (PHS-25CW Series bend atop pH/mv meter, Shanghai, China).

Weight variation

The weight variation was carried out as described by the British Pharmacopoeia 2013 [30]. Suppositories (20) selected from each batch were individually weighed, and the mean weight and per cent deviation was determined.

Melting point

The melting points of the suppositories were determined using the method of Coben and Lieberman [31].

Crushing strength

The crushing strength of the suppositories was determined using the hardness tester (R00060 model: EH01, DBK Instruments, Mumbai, India). Six suppositories were selected from each batch and the force under which each of the suppositories collapsed was recorded.

Disintegration time

The disintegration time for the suppositories in acetate buffer, pH 5.2, was determined as the time taken for the suppository to melt or disperse when immersed in a disintegration DBK test apparatus (Mumbai, India) maintained at 37 ± 0.5 °C [30].

In vitro release of clotrimazole from suppositories

In vitro drug release tests were carried out according to the USP XX basket method [21]. Each suppository was placed in the basket and lowered into a flask containing 500 ml of acetate buffer pH 5.2 rotated at a constant speed of 100 rpm and maintained at a constant temperature of 37 ± 0.5 °C. At specified time intervals, 5 ml samples were withdrawn and replaced with the same volume of fresh dissolution medium maintained at the same temperature. The amount of clotrimazole released was analysed spectrophotometrically at 259 nm using a UV–visible spectrophotometer (Spectrum lab 752 s UV–VIS spectrophotometer, Shanghai, China). The mean of three determinations was used in calculating drug release from each batch of suppositories. The clotrimazole release kinetics were investigated by fitting the release data into the Korsmeyer-Peppas equation using the DD Solver software programme (Microsoft Excel add-in, Excel 365 New Mexico, USA).

Assessment of antimycotic activities

The antimycotic activity was assessed using the agar-well diffusion technique. The prepared agar medium was autoclaved at 121 °C for 20 min. Using the McFarland dilution, cultures of C. albicans and C. spp were inoculated in sterile water. This was flooded onto the plated Sabouraud dextrose agar using a sterile swab. A sterile 6 mm cork borer was employed to bore 7 wells into each plate of solidified media. The clotrimazole-loaded suppository (100 mg) was suspended in 10 ml acetate buffer pH 5.2 and shaken at room temperature at 50 rpm. Samples were filled into the different wells using sterile syringes, under laminar airflow. The same was repeated for clotrimazole, CO, and coconut oil diluted to 50% with 1% ethanol. The antimycotic activity of the combination of clotrimazole and CO was carried out at ratios 1:1 and 1:2. The zones of inhibition around the wells were examined after 72 h at 25 °C, and their diameters were measured [32].

Stability study

The vaginal suppositories were kept at room temperature (30 ± 2 °C) and in the refrigerator (4 °C) for 12 weeks. The suppositories were assessed for their visual appearance—colour, odour, and consistency.

Data presentation and analysis

All determinations were conducted in triplicate and the data is presented as mean ± SD. Statistical analysis was performed using one-way Analysis of Variance (ANOVA) using GraphPad Prism® 5 (GraphPad Software Inc., San Diego, USA). Values with p < 0.05 were regarded as significant. In addition, the drug release mechanism was analysed using the DD Solver (Microsoft Excel add-in, Excel 365).

Results

The physicochemical, mechanical, and drug release properties, as well as correlation coefficients and n obtained by fitting the release data into the Korsmeyer-Peppas kinetic model, of the vaginal suppositories, are presented in Table 2. The pH of the vaginal suppositories ranged from 4.08 to 5.98, while their weight was generally between 0.883 and 1.220 g. The melting point generally ranged from 40 to 60 °C and the crushing strength was 0.46–37.98 N.

Table 2 Physicochemical properties and release kinetics of the vaginal suppositories using the Korsmeyer–Peppas model
Full size table

The disintegration or liquefaction time, followed the rank order of CPEG < CBT20S20C < CBS20C < CBSC < CBT20C < CBC1 < CBC2 < CBC3 < CCB < CBC4, indicating that CPEG vaginal suppositories had the lowest disintegration time, while CBC4 vaginal suppositories took the longest time to liquefy. The plots of % clotrimazole released over time for suppositories containing coconut oil solidified with beeswax, reference bases, and surfactants are shown in Figs. 1, 2 and 3, respectively. The time taken for 80% clotrimazole release (t80) is shown in Table 2. The results showed that CBT20C vaginal suppositories showed the fastest release while CCB exhibited the slowest.

Fig. 1
figure 1

Plots of percentage clotrimazole released against time (min) for the vaginal suppositories containing coconut oil and beeswax

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Fig. 2
figure 2

Plots of percentage clotrimazole released against time (min) for the vaginal suppositories containing either cocoa butter or PEG

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Fig. 3
figure 3

Plots of percentage clotrimazole released against time (min) for the vaginal suppositories containing coconut oil and surfactants

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The antimycotic activity of the coconut oil and the API (clotrimazole) tested, singly and in combination are presented in Table 3 and the antimycotic activities of the vaginal suppositories are presented in Table 4. Generally, antimycotic activity increased with an increase in concentration of the clotrimazole in the formulation, with higher activity against C. albicans.

Table 3 Antimycotic Activities of API and Coconut Oil
Full size table
Table 4 Antimycotic activity of the suppositories using Zone of inhibition (mm)
Full size table

The stability test showed that there was no change in the colour, odour, and consistency of the vaginal suppositories after 12 weeks.

Discussion

pH is a measure of the acidity or basicity of a medium, and the pH of the vaginal suppositories was acidic to mildly acidic with pH ranging from 4.08 to 5.98. The vaginal pH ranges from 3.8 to 5.0 [33]. This acidic pH is important in protecting the vaginal mucosa from pathogenic organisms [34].

The clotrimazole suppositories all had a good appearance and passed the weight uniformity test i.e. they met the BP specification for weight uniformity, which is a permissible percentage deviation of 5% [30].

Coconut oil in the tropics is liquid (room temperature being 30 ± 2 °C), hence the need to solidify it with beeswax. The melting point ranges of the vaginal suppositories indicate that none of the suppositories had melting points close to the body temperature, rather they had a general melting point range of 40–60 °C. This was probably because beeswax has a melting point of 62–65 °C, thus minimizing the effect of coconut oil [35, 36]. Suppositories prepared with cocoa butter had the lowest melting temperature while PEG had the highest because of its molecular weight. Oily bases have been known to melt at body temperature while aqueous bases disperse in the vaginal fluid. The majority of the time, melting and dispersion occur simultaneously in medicated suppositories depending on the base used and the drug present in the formulation [37]. The melting range also decreased with the addition of surfactants with suppositories containing the combined surfactants exhibiting lower melting temperatures than those containing a single surfactant. The melting point range of those without surfactants increased with an increase in the concentration of beeswax, suggesting that the presence of beeswax did influence the melting point ranges.

The crushing strength of suppositories is used to determine their mechanical strength during handling, packaging, and transportation. Although there is no set standard for what constitutes acceptable or “good” crushing strength levels for suppositories, the range is generally considered to be between 17.7 and 19.6 N [38]. Even though most vaginal suppositories showed lower crushing strength, it was observed that they remained solid and non-brittle at room temperature for the duration of the investigation, indicating that they may endure the mechanical strains of handling and transit. The crushing strength of the clotrimazole suppositories increased as the concentration of beeswax increased, and coconut oil decreased. Suppositories prepared with either Tween 20 or Span 20 had the lowest crushing strength (0.59 ± 0.05 and 0.53 ± 0.07, respectively), while the ones prepared from PEG had the highest crushing strength (32.56 ± 5.42, p < 0.05). According to previous reports, surfactants promote the spread of the melted suppository, increasing the contact surface, decreasing the viscosity of the molten mass, and decreasing the pathway of drug particles to the interface. Thus, it is expected that the suppositories in which they are present will be softer [39].

The disintegration time, which is the same as liquefaction has been used in this study; oily bases have been known to liquefy, and aqueous bases such as PEG disperse during the disintegration test [38]. Clotrimazole suppositories containing surfactants and the ones prepared from PEG had significantly lower disintegration/liquefaction time (p < 0.05) in the rank order CPEG < CBTS20C < CBS20C < CBSC < CBT20C, than suppositories without surfactants and those prepared with cocoa butter, which ranged between 305 and 388 min. The lower disintegration/liquefaction time of suppositories with surfactants was expected as they had very low crushing strengths. The combination of Tween 20 and Span 20 significantly reduced the liquefaction time of the vaginal suppositories, suggesting improved drug release. For suppositories without surfactants, liquefaction time increased with an increase in the concentration of beeswax and a decrease in the concentration of coconut oil.

Clotrimazole release from the suppositories followed the rank order CPEG > CBC3 > CBC1 > CBS20C > CBC4 > CBT20C > CBC2 > CCB > CBTS20C > CBSC. This shows that suppositories made from PEG had the fastest drug release, possibly because clotrimazole is lipophilic in nature and thus, it does not bind to PEG; a water-miscible base, unlike in the case of cocoa butter in which its release was poor [40]. Besides, PEG is known to improve the solubility of therapeutic agents [41]. The presence of beeswax did not hinder drug release and the addition of surfactants did not appear to greatly improve the release of clotrimazole. Surfactants increase drug release by reducing the interfacial tension between mucosal fluids and the suppository base [41]. Interestingly, drug release from the vaginal suppositories without the surfactants did not show a clear trend despite the correlation between their crushing strength and disintegration time. Based on the t80, the ranking of drug release from the formulation was from CBT20C > CBC4 > CPEG > CBS20C. Thus, showing that clotrimazole partitioned through the oil/water interface fastest through CBT20C and CBC4. It has been reported that drug release from suppositories occurs through the initial melting, spreading of the melted mass, settling of the drug particles, movement of the drug particles through the oil/water interface, and dissolution of the drug particles in the mucosal fluid [21, 42]. Drug release from all the vaginal suppositories had regression coefficients, r2 ≥ 0.887 and diffusion constant, 0.5 < n < 1 (0.480–0.945), indicating non-Fickian anomalous diffusion i.e. both diffusion and relaxation (erosion) for CPEG, CBTS20C, CBSC, CCB, CBC1 and CBC3 while it was n > 1 (1.144–1.538) for CBC2, CBT20C, CBC4 and CBS20C, which is Super Case II transport mechanism (relaxation), meaning that drug release accelerated towards the endpoint of the drug release [43].

The antimycotic properties indicate that the zone of inhibition produced against C. albicans and Candida spp by the combination of the coconut oil and the API was higher than the activities of the coconut oil and API singly, thus suggesting a synergistic effect. In the suppository formulations, the rank of the zone of inhibition against C. albicans was in the order CPEG > CCB > CBS20C > CBC4 > CBT20S20C > CBC3 = CBT20C > CBC2 > CBC1. The antimycotic activity also increased with an increase in the concentration of clotrimazole in the formulation. The antimycotic activities of the formulations against C. tropicalis and C. krusei, which are both implicated in VVC, were in the rank order of CBT20C > CPEG = CBSC = CBT20S20C > CBC4 = CCB = CBS20C > CBC3 > CBC2 > CBC1, and CBS20C > CBC4 = CPEG > CCB > CBT20C > CBC3 = CBT20S20C > CBC1 > CBC2, respectively.

It is impressive that CBC4 with the least concentration of coconut oil exhibited higher antimycotic activity and faster release (t50 and t80) of clotrimazole than the other suppositories without surfactants, but comparable activities to the known bases, PEG and cocoa butter. This suggests that the concentration of coconut oil in CBC4 (40%) might just be optimal for the formulation, as it had activity against the three Candida species and generally had better activity than cocoa butter. Coconut oil contains lauric and capric acid, which confer antimycotic properties on it thus synergizing the activity of clotrimazole [44].

Conclusion

The clotrimazole vaginal suppositories met BP specifications for weight uniformity and had a pH that conformed with vaginal pH. The addition of surfactants singly rather than in combination improved the rate of drug release. The presence of surfactants in the suppositories reduced disintegration time and enhanced drug release. The formulation containing 40% w/w coconut oil had comparable drug release properties and antimycotic activity with those prepared with PEG and cocoa butter. The optimized formulation with the desired physicochemical and drug release properties was obtained with coconut oil (40% w/w) solidified with beeswax (50% w/w) as a base. Coconut oil appeared to have a synergistic effect on clotrimazole. Thus, clotrimazole vaginal suppositories with coconut oil as a base in the treatment of vulvovaginal candidiasis showed potential against Candida species.

Availability of data and materials

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

Abbreviations

VVC:

Vulvovaginal candidiasis

OTC:

Over the counter

CO:

Coconut oil

CBC1:

Clotrimazole + beeswax 20% + coconut oil 70%

CBC2:

Clotrimazole + beeswax 30% + coconut oil 60%

CBC3:

Clotrimazole + beeswax 40% + coconut oil 50%

CBC4:

Clotrimazole + beeswax 50% + coconut oil 40%

CCB:

Clotrimazole + cocoa butter

CPEG:

Clotrimazole + polyethylene glycol

CBT20C:

Clotrimazole + Tween 20 + coconut oil

CBS20C:

Clotrimazole + Span 20 + coconut oil

CBSC:

Clotrimazole + sodium lauryl sulphate + coconut oil

CBTS20C:

Clotrimazole + Tween 20 + Span 20 + coconut oil

PEG:

Polyethylene glycol

t 80 :

Time taken to release 80% of the drug

t 50 :

Time taken to release 50% of the drug

API:

Active pharmaceutical ingredient

References

  1. Murina F, Lubrano C, Cappelli E, Campo M, Taraborrelli S (2023) The role of female intimate hygiene practices in the management of vulvovaginal candidiasis A randomized controlled open label trial. Health Care Women Int 44(5):689–700

    PubMed  Google Scholar 

  2. Asare KK, Bentil HA, Gyesi E, Amoah S, Bentsi-Enchill F, Opoku YK (2023) Candidiasis profile at the outpatient department of the university of cape coast hospital in the central region of Ghana: a retrospective study. BMC Women’s Health 23:1–10

    Google Scholar 

  3. Turner SA, Butler G (2014) The candida pathogenic species complex. Cold Spring Harb Perspect Med 4:a019778

    PubMed  PubMed Central  Google Scholar 

  4. Yano J, Sobel JD, Nyirjesy P, Sobel R, Williams VL, Yu Q, Fidel PL Jr (2019) Current patient perspectives of vulvovaginal candidiasis: incidence, symptoms, management and posttreatment outcomes. BMC Women’s Health 19:48

    PubMed  PubMed Central  Google Scholar 

  5. Sun Z, Ge X, Qiu B, Xiang Z, Jiang C, Wu J, Li Y (2023) Vulvovaginal candidiasis and vaginal microflora interaction: microflora changes and probiotic therapy. Front Cell Infect Microbiol 13:1123026

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Hall RA, Noverr MC (2017) Fungal interactions with the human host: exploring the spectrum of symbiosis. Curr Opin Microbiol 40:58–64

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Amorós M, Palma JCM, Esteve M, Salado M, Macatangga MG, Benítez GA, Cortés J (2023) Experience with a non-hormonal Centella asiatica, hyaluronic acid and prebiotic-based vaginal gel in women with recurrent vulvovaginal candidiasis: case studies. Clin Med Insights: Case Rep 16:1–4

    Google Scholar 

  8. Gonçalves B, Ferreira C, Alves CT, Henriques M, Azeredo J, Silva S (2016) Vulvovaginal candidiasis: epidemiology, microbiology and risk factors. Crit Rev Microbiol 42(6):905–927

    PubMed  Google Scholar 

  9. Fukazawa EI, Witkin SS, Robial R, Vinagre JG, Baracat EC, Linhares IM (2019) Influence of recurrent vulvovaginal candidiasis on quality of life issues. Arch Gynecol Obstet 300:647–650

    PubMed  Google Scholar 

  10. Denning DW, Kneale M, Sobel JD, Rautemaa-Richardson R (2018) Global burden of recurrent vulvovaginal candidiasis: a systematic review. Lancet Infect Dis 18(11):e339–e347

    PubMed  Google Scholar 

  11. Blomberg L, Backman K, Kirjavainen PV, Karvonen AM, Harju M, Keski-Nisula L (2023) Vulvovaginal yeast infections, gestational diabetes and pregnancy outcome. BMC Pregnancy Childbirth 23:70

    PubMed  PubMed Central  Google Scholar 

  12. He Y, Tang R, Deng J, Cai T, He P, Wu J, Cao Y (2022) Effects of oestrogen on vulvovaginal candidosis. Mycoses 65(1):4–12

    PubMed  Google Scholar 

  13. Sobel JD, Faro S, Force RW, Foxman B, Ledger WJ, Nyirjesy PR, Summers PR (1998) Vulvovaginal candidiasis: epidemiologic, diagnostic, and therapeutic considerations. Am J Obstet Gynecol 178(2):203–211

    CAS  PubMed  Google Scholar 

  14. Sobel JD (2016) Recurrent vulvovaginal candidiasis. Am J Obstet Gynecol 214(1):15–21

    PubMed  Google Scholar 

  15. Mahant S, Sharma AK, Gandhi H, Wadhwa R, Dua K, Kapoor DN (2022) Emerging trends and potential prospects in vaginal drug delivery. Curr Drug Deliv 20(6):730–751

    Google Scholar 

  16. Sobel JD (1998) Vulvovaginitis: when Candida becomes a problem. Dermatol Clin 16(4):763–768

    CAS  PubMed  Google Scholar 

  17. Sobel JD (1999) Limitations of antifungal agents in the treatment of Candida vaginitis: future challenges. Drug Resist Updates 2(3):148–152

    CAS  Google Scholar 

  18. Ksiezopolska E, Gabaldón T (2018) Evolutionary emergence of drug resistance in candida opportunistic pathogens. Genes 9(9):461

    PubMed  PubMed Central  Google Scholar 

  19. Mathema B, Cross E, Dun E, Park S, Bedell J, Slade B, Perlin DS (2001) Prevalence of vaginal colonization by drug-resistant candida species in college-age women with previous exposure to over-the-counter azole antifungals. CID. Brief Rep 33:e24

    Google Scholar 

  20. Kalmár E, Lasher JR, Tarry TD, Myers A, Szakonyi G, Dombi G, Alexander KS (2014) Dosage uniformity problems which occur due to technological errors in extemporaneously prepared suppositories in hospitals and pharmacies. Saudi Pharm J 22(4):338–342

    PubMed  Google Scholar 

  21. Akin-Ajani OD, Odeku OA, Babalola Y (2019) Formulation of paediatric paracetamol suppositories using shea butter and dika fat as suppository bases. Trop J Nat Prod Res 3(2):31–36

    CAS  Google Scholar 

  22. Akin-Ajani OD, Awe T, Adediran KA (2023) Preliminary investigation of Ciprofloxacin-loaded microparticles for the treatment of bone diseases using coconut oil and shea butter. Ger J Pharm Biomater 2(1):18–25

    Google Scholar 

  23. Odoom W, Edusei VO (2015) Evaluation of saponification value, iodine value and insoluble impurities in coconut oils from Jomoro District of the Western Region of Ghana. Asian J Agric Food Sci 3(5):494–499

    Google Scholar 

  24. Seneviratne K, Jayathilaka N (2016) Coconut oil: chemistry and nutrition. 2016. Lakva Publishers, Battaramulla, p 130

    Google Scholar 

  25. Kamel R, Abbas H (2018) Rational for the use of coconut oil-based anti-mycotic pessaries to combat recurrent vaginal infection: in-vitro/in-vivo evaluation and preliminary prospective clinical investigation. Int J Appl Pharm 10:159–166

    CAS  Google Scholar 

  26. Ogbolu DO, Oni AA, Daini OA, Oloko A (2007) In vitro antimicrobial properties of coconut oil on Candida species in Ibadan, Nigeria. J Med Food 10(2):384–387

    CAS  PubMed  Google Scholar 

  27. Deen A, Visvanathan R, Wickramarachchi D, Marikkar N, Nammi S, Jayawardana BC, Liyanage R (2021) Chemical composition and health benefits of coconut oil: an overview. J Sci Food Agric 101(6):2182–2193

    CAS  PubMed  Google Scholar 

  28. Crowley PD, Gallagher HC (2014) Clotrimazole as a pharmaceutical: past, present and future. J Appl Microbiol 117(3):611–617

    CAS  PubMed  Google Scholar 

  29. Shaveta S, Harpreet K, Jyoti S, Rahul Y (2022) A short review on liquisolid technology in anti-fungal drugs. World J Pharm Sci 10(01):144–150

    Google Scholar 

  30. British Pharmacopoeia C (2012) British pharmacopoeia 2013. Stationery Office, London

    Google Scholar 

  31. Coben LJ, Lieberman HA. Suppositories. In: Lachman L, Lieberman HA, Kanig J (Eds) The theory practices of industrial pharmacy, Lea and Febiger Philadelphia, pp 564–588

  32. Akin-Ajani OD, Ajala TO, Ogunnubi MA (2020) Formulation and antimicrobial evaluation of isopropyl hand sanitizer using co-processed excipients. Niger J Pharm Res 16(1):9–20

    Google Scholar 

  33. Szymańska E, Wojasiński M, Dąbrowska J, Krzyżowska M, Nowicka M, Ciach T, Winnicka K (2022) Chitosan-poly(ethylene oxide) nanofibrous mat as a vaginal platform for tenofovir disoproxyl fumarate: the effect of vaginal pH on drug carrier performance. Int J Biol Macromol 222(Pt A):856–867

    PubMed  Google Scholar 

  34. Linhares IM, Summers PR, Larsen B, Giraldo PC, Witkin SS (2011) Contemporary perspectives on vaginal pH and lactobacilli. Am J Obstet Gynecol 204(2):120.e121-125

    Google Scholar 

  35. Al-Shehri BM, Haddadi T, Alasmari ME, Ghramh HA, Khan KA, Mohammed MEA, Khayyat MM (2022) Effect of storage time and floral origin on the physicochemical properties of beeswax and the possibility of using it as a phase changing material in the thermal storage energy technology. Foods 11(23):3920

    CAS  PubMed  PubMed Central  Google Scholar 

  36. Sahu S, Rathi JC, Sharma MR (2021) Formulation and evaluation of multiple emulsion of clotrimazole. World J Pharmaceut Res 10(12):1233–1246

    CAS  Google Scholar 

  37. Baviskar P, Bedse A, Sadique S, Kunde V, Jaiswal S (2013) Drug delivery on rectal absorption: suppositories. Int J Pharm Sci Rev Res 21(1):70–76

    Google Scholar 

  38. Aremu OI, Adjuzie JP, Olayemi OJ, Okoh JE, Ekere KE, Fatokun OT, Emeje MO (2019) Formulation and evaluation of acetylsalicylic acid suppositories using capra hircus (Goat) fat and its binary blends. ACTA Pharm Sci 57(4):77–91

    CAS  Google Scholar 

  39. Realdon N, Zotto MD, Morpurgo M, Franceschinis E (2008) Effects of surfactant characteristics on drug availability from suppositories. Die Pharm Int J Pharm Sci 63(6):459–463

    CAS  Google Scholar 

  40. Ausili A, Yakymenko I, Teruel JA, Gómez-Fernández JC (2021) Clotrimazole fluidizes phospholipid membranes and localizes at the hydrophobic part near the polar part of the membrane. Biomolecules 11(9):1304

    CAS  PubMed  PubMed Central  Google Scholar 

  41. Allen LV Jr (2021) Basics of compounding: excipients used in nonsterile compounding, part 10: rectal suppository bases. Int J Pharm Compd 25(4):304–309

    PubMed  Google Scholar 

  42. Moes AJ (1989) Suppositories formulation and drug release. Boll Chim Farm 128(1):5–12

    CAS  PubMed  Google Scholar 

  43. Akin-Ajani OD, Ikehin MM, Ajala TO (2019) Date mucilage as co-polymer in metformin-loaded microbeads for controlled release. J Excip Food Chem 10(1):3–12

    Google Scholar 

  44. Abbas AA, Assikong EB, Akeh M, Upla P, Tuluma T (2017) Antimicrobial activity of coconut oil and its derivative (lauric acid) on some selected clinical isolates. Int J Med Sci Clin Invent 4(8):3173–3177

    Google Scholar 

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Acknowledgements

The authors acknowledge the Alexander von Humboldt Foundation, Bonn, Germany for the donation of equipment to OAO’s Drug Delivery Laboratory and the assistance of the technologists at the Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.

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Authors and Affiliations

  1. Department of Pharmaceutics and Industrial Pharmacy, University of Ibadan, Ibadan, Nigeria

    Ayomide D. Mustapha, Folusho M. Oyedepo, Olufunke D. Akin-Ajani & Oluwatoyin A. Odeku

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  1. Ayomide D. Mustapha
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  2. Folusho M. Oyedepo
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  3. Olufunke D. Akin-Ajani
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  4. Oluwatoyin A. Odeku
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Contributions

OAO contributed to the design of the study; FMO and ADM collected the samples and performed the experiments; ODA analysed the data and drafted the paper; all authors read and approved the final manuscript.

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Correspondence to Olufunke D. Akin-Ajani.

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Mustapha, A.D., Oyedepo, F.M., Akin-Ajani, O.D. et al. Design and evaluation of antifungal vaginal suppository using coconut oil as base for vulvovaginal candidiasis. Futur J Pharm Sci 9, 80 (2023). https://doi.org/10.1186/s43094-023-00533-w

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