Association of vitamin C status in diabetes mellitus: prevalence and predictors of vitamin C deficiency
Future Journal of Pharmaceutical Sciences volume 6, Article number: 30 (2020)
Vitamin C is one of the most important micronutrient required for various physiological roles in the human system. Evidences suggest that there is an inadequate status of vitamin C in diabetes mellitus. The objectives of this study is to understand the prevalence of vitamin C deficiency in established type II diabetes mellitus patients and to study the correlation between various variables of diabetes mellitus with serum vitamin C levels.
A prospective cross-sectional study to assess the prevalence of vitamin C deficiency was carried out in diabetes patients. Fasting blood sugar levels, glycated hemoglobin, serum malondialdehyde levels, and lipid profile levels were correlated with serum vitamin C levels.
The prevalence rate of hypovitaminosis C is found to be 55.13% among the enrolled patients. There is a significant increase in the systolic blood pressure levels in patients with inadequate as well as deficient vitamin C levels (p < 0.05). Inverse relationship exists between fasting blood sugar and vitamin C levels (p < 0.001). Similarly total cholesterol levels were also inversely related to the vitamin C levels (p = 0.0031). Body mass index, glycated hemoglobin, and fasting blood sugar levels are important predictors of vitamin C deficiencies.
Vitamin C deficiency is well established among diabetes mellitus patients. Deficiency of vitamin C levels has an impact on the serum malondialdehyde levels suggesting increased oxidative stress. The higher oxidative stress would have led to increase in glycated hemoglobin. Further research must be carried out to understand the beneficial effects of vitamin C supplementation in diabetes mellitus.
The role of ascorbic acid in various metabolic diseases has been researched upon ever since its discovery. Ascorbic acid is an important vitamin that has well established biological role due its antioxidant nature. The human population is unable to produce their own ascorbic acid, and hence, it must be strictly obtained from the dietary sources . Vitamin C rich foods are pretty common and regularly consumed in our daily diet . Inadequacy occurs due to the excessive consumption of vitamin C in destabilizing the free radicals. In all metabolic illnesses such as diabetes, there is an inadequate vitamin C serum concentration.
One of the most common diseases affecting the global population is diabetes mellitus. Diabetes mellitus has an extensive pathology especially the mechanism involving oxidative stress is still complex. The generation of the reactive oxygen species (ROS) such as superoxide anions could damage the islets of Langerhans particularly the β cells resulting in reduced insulin release. These ROS affects the secondary signal transducers such as protein kinase C . The activation of such signal transducers interfere with insulin activity leading to the development of insulin resistance. Studies have suggested vitamin C deficiency is far more prevalent in pre-diabetics and established diabetes mellitus patients when compared to non-diabetic individuals .
There are several reports that stated the use of ascorbic acid as a supplementation in the prevention of disease progression and their complications . The objectives of this study is to understand the prevalence of vitamin C deficiency in established type II diabetes mellitus patients, to study the correlation between various variables of diabetes mellitus with serum vitamin C levels.
A prospective cross-sectional study was carried out in a tertiary care teaching hospital between September 2018 and August 2019. Sample size was calculated using Raosoft online calculator. With a population size of 1200 patients (derived from the prevalence data of the hospital in the previous year prior to the study) and a confidence interval of 95%, the sample size was calculated to be 292 . The study was approved by the institutional ethics committee VISTAS-SPS/IEC/2018/1/07. All the study participants have provided a written informed consent for their willingness to participate in this study.
Patients who are 18 years or older and diagnosed with type II diabetes mellitus characterized by a fasting blood sugar level between 126 mg/dL and 250 mg/dL and glycated hemaglobin level below 9% were included for the study as higher level may require multiple drug therapy. Patients who are only on metformin were included as other oral hypoglycemic agents may affect the metabolism of ascorbic acid . Participants who were unable to give informed consent, patients who had previous experience of cardiovascular events were excluded. Pregnant and nursing women were also excluded from the study.
A specially designed data extraction form is used to obtain the preliminary data such as demographics like age, sex, family history of diabetes mellitus, qualification, smoking history, alcoholism, duration of diabetes, and past medical history were recorded. Anthropometric measurement such as height and body weight was recorded, and they were used to determine the body mass index (BMI). The glycemic status parameters such as fasting blood glucose (FBS) and glycated hemoglobin (HbA1C) were recorded using conventional methods.
Cardiovascular risk assessment parameters such as systolic blood pressure (SBP), high density lipoprotein (HDL), total cholesterol (TC), and triglycerides (TG) were analyzed. From these available data, Framingham Risk Score was calculated. Framingham Risk Score is a Canadian risk assessment score for 10-year cardiovascular risk projection. Oxidative stress markers such as malondialdehyde levels were also analyzed.
Serum vitamin C levels were analyzed from the blood which was acidified with a chelator and perchloric acid and was subjected to automated high performance liquid chromtography (HPLC).
The study participants were counseled by a certified dietician of the hospital to follow a standard diet with required daily allowance of vitamin C. The participants were asked to maintain a patient’s diary to track the food consumed prior to the collection of blood.
Descriptive statistics were used to analyze the preliminary data in the form of mean ± SEM. The baseline parameters were analyzed using one-way ANOVA and chi-squared test as appropriate with a significance level of 95%. Pearson correlation was used to correlate the association between each variable in a cohort with serum vitamin C levels. All the univariate analyses used a p < 0.05 to be considered statistically significant. The statistical analyses were carried out using IBM SPSS (Statistical Programme for social Sciences version 22) and GrapPad prism version 9.0.
Initially, 359 patients were screened and 292 participants were selected for the study based on the selection criteria participants. The patients were categorized to three groups depending upon their serum vitamin C levels such as sufficient (serum vitamin C > 0.6 mg/dL), Insufficient (serum vitamin C 0.6 to 0.3 mg/dL) and deficient (serum vitamin C < 0.3 mg/dL).
It could be inferred from the table 1 that the prevalence rate of hypovitaminosis C is 55.13%. There is a significant high systolic blood pressure levels in inadequate as well as deficient groups is seen.
It is evident from Table 2 that cardiovascular risk markers and oxidative stress markers are significantly altered in patients with vitamin C deficiency.
It can be seen from Table 3 that a significant inverse relationship exists between fasting blood sugar and vitamin C levels. Similarly total cholesterol levels were also inversely related to the vitamin C levels.
On performing the Pearson correlation between glycated hemoglobin and serum vitamin C levels, r = − 0.8741**. When correlation analysis was performed between malondialdehyde levels and serum vitamin C levels, r = − 0.8121**.
The glycated hemoglobin has a significant inverse relationship with serum vitamin C levels (Fig. 1). Malondialdehyde which is predominantly scavenged by anti-oxidants has also a significant inverse relationship with vitamin C levels (Fig. 1).
Although smoking status is an important predictor for vitamin C deficiencies, it is not significant in the results obtained. Body mass index, glycated hemoglobin, and fasting blood sugar levels are important predictors of vitamin C deficiencies (Table 4).
It has been observed from our study that there is a prevalence rate of 55.13% hypovitaminosis C (deficient vitamin C status) in type 2 diabetes mellitus patients which is similar to the study by David et al. . Vitamin C deficiency may further contribute to the microvascular and microvascular complications in diabetes mellitus patients [9, 10]. It could be seen from our study that lipid profile parameters are significantly higher in the vitamin c deficiency group. Oxidative stress and the impact of reactive oxygen species level contribute to the higher levels of lipids in vitamin C deficiency status [4, 11].
The study showed that there is a significant inverse relationship between the individual predictors such as fasting blood sugar, glycated hemoglobin, high-density lipoproteins, total cholesterol, body mass index, triglycerides, malondialdehyde levels, and vitamin C levels.
Body mass index levels were inversely proportional to the serum vitamin C concentration. Patients with higher body mass index require higher consumption of ascorbic acid for the regular needs . The fasting blood sugar is inversely proportional to the serum vitamin C levels which is in accordance with the previous results [13, 14].
Malondialdehyde levels are also inversely related to their serum vitamin C levels. In diabetes mellitus, there is an increased impact of reactive oxygen species and free radicals; therefore, there is an increase demand of the antioxidants to scavenge the free radicals . However, the individual consumption must be increased in order to overcome these deficiencies.
Supplementation of vitamin C in diabetes mellitus patients may be beneficial. Vitamin C being water soluble vitamin is at lower risk of hypervitaminosis C when compared to other antioxidant vitamins such as vitamin E which is lipid soluble [10, 16]. Certain studies predicted that vitamin C is being excreted through urine . This should be analyzed in human also in the future. Further research is required to assess the beneficial effects of vitamin C supplementation in diabetes mellitus patients in improvement of their glycemic control and prevention of their complications through this potent antioxidant.
There is a definite prevalence of vitamin C deficiency in type 2 diabetes mellitus patients. Also, there is an inverse relationship between the fasting blood sugar levels, body mass index, and vitamin C levels. Significant negative correlation has been observed between glycated hemoglobin levels with serum vitamin C levels. Vitamin C is an important antioxidant vitamin that is necessary for scavenging of these free radicals and prevention of further complication of diabetes mellitus. There is a necessity to increase the supply of vitamin C to diabetes mellitus patients as they may be beneficial in managing the glycemic control and preventing further complications.
Availability of data and materials
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
Analysis of variance
Body mass index
Fasting blood sugar
High performance liquid chromatography
Reactive oxygen species
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The authors are thankful to Vels Institute of Science Technology and Advanced Studies and its management for extending their support and research facilities.
Ethics approval and consent to participate
The study was approved by the institutional ethics committee VISTAS-SPS/IEC/2018/1/07. All the study participants have provided a written informed consent for their willingness to participate in this study.
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A consent for publication was obtained from all participants.
The authors declared that they have no competing interests.
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D, P., Puvvada, R. & M, V. Association of vitamin C status in diabetes mellitus: prevalence and predictors of vitamin C deficiency. Futur J Pharm Sci 6, 30 (2020). https://doi.org/10.1186/s43094-020-00040-2