Skip to main content

Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L.



Piper nigrum L. is one of the widely used herbs in Ayurvedic medicine. Piperine is a major phytoconstituent that is responsible for most of the activity of the herb. Quality assessment and standardization of such phytoconstituents is the need of the hour. The present study aims at developing a Quality by design (QbD)-based RP-HPLC Method for marker-based standardization of Piper nigrum L. fruits along with its quality assessment.


The quality assessment of the crude sample was carried out by evaluating pharmacognostic parameters and analysis of toxic contaminants. The analytical target profile and critical quality attributes were determined and 22 factorial design was employed for optimization of the method. By performing the experiments as per the QbD concept the optimized mobile phase was identified as Acetonitrile and Water with 0.05% Acetic acid in the ratio of 70:30, with a flow rate of 1 mL/min and UV detection at 342 nm. The retention time of Piperine was found to be 5.5 min and the amount of Piperine in crude P. nigrum fruits and its extract was found to be 3.6% w/w 5.62% w/w, respectively. The Pharmacognostic parameters showed the results within specified limits and the crude drug sample showed the absence of toxic contaminants in it thus indicating the purity of the drug.


The utilization of the QbD approach leads to the development of a more precise and reliable method for the quantification of phytocompounds.


Herbal drugs have been used in medical practice for many years and are gaining considerable momentum in the world during the past decades [1]. As the requirements of herbal drugs are increasing worldwide, their quality control and standardization have become more imperative. Since quality control and standardization of herbal drugs is an important task with great challenges; factors such as geographic and environmental differences of growing conditions, physical constants, adulterations, microbiological contamination, and foreign materials could affect the quality and also batch-to-batch uniformity of herbal products. Hence quality assessment of raw material concerning pharmacognostic and phytochemical parameters is essential in order to prove the identity and purity of herbal drugs [2, 3].

Chemical marker-based standardization is a widely accepted method for the quality control of herbal drugs. In these methods, suitable markers or pharmacologically active compounds in the herb are analyzed by various chromatographic techniques for evaluating the quality and authenticity of herbal medicines. Several chromatographic techniques ranging from simplest Thin Layer Chromatography to sophisticated High-Performance Liquid Chromatography (HPLC), High-Performance Thin Layer Chromatography (HPTLC), and Gas Chromatography (GC) can be utilized for such marker-based standardization of herbal drugs [4].

Along with the utilization of marker-based techniques, the application of novel quality approaches are essential for quality assessment and development standardization parameters for herbal drugs. In recent times, pharmaceutical companies adopting Quality by Dsign (QbD) as a fundamental pharmaceutical quality model [5]. Quality by Design is defined as “a systematic approach to development that begins with predefined objectives and emphasizes product and process understanding based on sound science and quality risk management” [6]. According to the recent literature the QbD concept can serve as a novel approach for quality control of herbal drugs [7,8,9].

Application of the QbD approach in analytics is one of the alternatives which reduces the experimental time and cost for drug analysis. The QbD approach suggests looking into the quality of the analytical process during the development stage itself. Analytical QbD explores the scientific understanding of method variables and their interactions, finally provides a region for a highly robust and cost-effective approach [3, 10, 11].

Piper nigrum L. belongs to the family Piperaceae and is known as the Black pepper or king of spices. Black pepper fruits are the source of one of the world's most widely and frequently used spices. The fruits of the plant have a long history of usage in Ayurvedic and folklore medicine, particularly for digestive ailments [12]. The main chemical constituents of fruits are alkaloids, among the alkaloid content Piperine is a major phytoconstituent that is responsible for most of the activity of the herb. In Ayurvedic medicine, black pepper has been used to aid digestion, improve appetite, treat coughs, colds, breathing and heart problems, colic, diabetes, anemia, and piles. It improves drug availability and is used as a bio enhancer due to its ability to enhance the efficacy of other drugs [13, 14]. In the present research work, an attempt has been made to develop quality control standards for P. nigrum fruits by carrying out the pharmacognostic evaluation along with chemical marker-based standardization of P. nigrum fruits by application of the Analytical QbD approach.



Standard Piperine was provided as a gift sample by the Himalaya drug company, Bengaluru India. Acetonitrile and water of HPLC grade purchased from Merck, Mumbai, India Pvt Ltd. Other chemicals and reagents used in the research work were of analytical grade.

Plant material and processing

Sample of crude Piper nigrum L. fruits was procured and authenticated from Shri B. M. Kankanwadi Ayurveda Mahavidyalaya, Karnataka. The fruits were shade dried and were coarsely ground into homogenous powder using a mechanical grinder and stored at room temperature. The extraction of the crude drug was carried out by cold maceration followed by the soxhlet extraction method. Ethanol and Water in the ratio of 90:10 was used for the extract preparation.

Quality assessment of P. nigrum fruits

The quality of P. nigrum fruits was assessed by evaluating the quality control parameters mentioned in WHO guidelines. Physico-chemical parameters including moisture content, extractive value, and ash value were performed along with phytochemical analysis [15, 16]. The crude sample of P. nigrum fruits were further analyzed for the determination of toxic substances such as Aflatoxins, pesticide residues, and heavy metals. Aflatoxins were determined by HPLC method as per the standard procedure [17]. Aflatoxins B1, B2, G1, and G2 were analyzed in the powdered sample. Analysis of pesticide residue was carried out by Gas Chromatography–Mass spectroscopy (GC–MS) Instrument. The presence of a total of 17 pesticide contaminants was analyzed in crude powdered fruits. And the presence of heavy metals was analyzed by Atomic Absorption Spectroscopy. Heavy metals, namely lead, cadmium, arsenic, mercury, and chromium were tested in the crude powdered sample.

Instrumentation and chromatographic conditions

HPLC system (Agilent technologies 1220 Infinity II LC) used for the analysis consisted of a system controller, low-pressure gradient pump, solvent delivery module, online degasser, manual sample injector (injection volume ranging between 5 and 20 µL), and UV–Vis detector. A Reversed-phase C-18 column (5 µm, 4.6 mm, 250 mm, ZORBAX) was used for chromatographic separation. The mobile phase was composed of acidic Water adjusted with Acetic acid, and Acetonitrile in different ratios. Samples were analyzed at the flow rate of 1 mL/min and the detection wavelength was set at 342 nm. For each analysis, a 20 µL sample was injected into the column.

Preparation of standard and sample solution

A stock of solution of standard Piperine (1 mg/mL) was prepared by dissolving accurately weighed 10.00 mg of Piperine in 10.00 ml of HPLC grade methanol with the help of a sonicator. Further working standard solutions were prepared by diluting the stock solution with the mobile phase.

For the preparation of the sample, accurately weighed 10 mg of crude powdered sample and extract of P. nigrum fruits was transferred to 10.00 mL volumetric flask individually containing 5.00 mL of methanol. The methanolic solution was sonicated for 15.00 min to ensure the complete dissolution of piperine. The volume was made up to 10.00 mL with methanol and was used for further analyses. The solution was filtered through a 0.25 µm membrane filter prior to their injection into the chromatographic column.

Analytical Quality by design assisted based HPLC method development

Defining of analytical target profile (ATP) and critical quality attributes (CQA)

Defining of Analytical Target Profile (ATP) is the first step in the Analytical QbD. ATP serves as the quality specification of the analytical method which should be achieved so as to attain reliable results. Critical Quality Attributes (CQA’s) are the quality characteristics related to method performance. CQAs have to be identified from the defined ATP which will be useful in ascertaining the satisfactory performance of the developed method [10, 18].

Optimization of method using design of experiments (DoE)

The optimization of the analytical method was typically performed on parametric variables using Design of Experiments (DoE) to ensure that maximum understanding is gained while minimizing the total number of experiments. A simple 22 full factorial design with 2 factors and 2 levels, resulting in 4 experimental runs was employed in order to identify the optimized analytical conditions. The DoE was developed using Design-Expert software version 12.0, (Stat-Ease Inc., Minneapolis, MN, USA).

Establishment of method operable design region (MODR)

After performing the experimental runs planned as per 22 factorial design, the obtained data was studied in terms of regression models and factor-response relationship, to generate the Method Operable Design Region (MODR). From the established MODR the optimized chromatographic conditions were predicted based on the specified target or goals of each CQA in terms of overlay plot. Within the design region, all the specifications mentioned in the ATP are fulfilled at a specified risk level.

Validation of the optimized method

Validation of the optimized RP-HPLC method was performed as per ICH Q2 (R1) guidelines [19]. The described method was extensively validated in terms of system suitability, linearity, LOD, LOQ, Intra-day precision, Inter-day precision, and accuracy.


Quality assessment of P. nigrum fruits

Quality assessment of herbal drugs is one of the most essential and crucial tasks which enables the determination of quality and safety of the crude drug. Evaluation of physicochemical properties serves as a tool for quality control and identification of crude drugs. Physicochemical parameters such as the moisture content, aqueous soluble extractive value, alcohol soluble extractive value, total ash value, acid insoluble ash value, and water-soluble ash value was found to be 7.6 ± 0.69, 8.67 ± 0.58, 9.83 ± 0.76, 3.83 ± 0.29, 0.45 ± 0.21, and 2.67 ± 0.29% w/w, respectively.

The preliminary phytochemical analysis gives a brief idea about the presence of various secondary metabolites in medicinal plant materials. The preliminary phytochemical analysis of P. nigrum fruits revealed the presence of important secondary metabolites such as alkaloids, flavonoids, tannins, and steroids whereas phenols, saponins, and glycosides were found to be absent.

Determination of toxic substances in herbal drugs is one of the major criteria for the assessment of quality in herbal drugs. Determination of Aflatoxins, heavy metals, and pesticide residue is a prerequisite criterion for the export of herbal drugs to foreign countries. Hence, this serves as a vital measure for defining the quality of herbal drugs or products [20]. The HPLC chromatogram obtained from the Aflatoxins analysis in the sample is depicted in Fig. 1. Our earlier study has reported the retention time for Aflatoxins standards B1, B2, G1, and G2 as 13.70, 11.35, 9.08, and 7.65 min, respectively [11]. From the sample chromatogram, it can be observed that no significant peaks are obtained at the above mentioned retention times, indicating the absence of Aflatoxins in the crude P. nigrum sample. Further, the pesticide residues and heavy metals in the sample were found to be below the limit of quantification. Where, the quantification limit for pesticide residues, lead, cadmium, mercury, arsenic and chromium being 0.01 mg/kg, 1.1 mg/kg, 0.5 mg/kg, 0.1 mg/kg, 0.1 mg/kg, and 0.5 mg/kg, respectively.

Fig. 1
figure 1

HPLC chromatograms for (A) standard aflatoxins and (B) P. nigrum sample

Initial method development

Initial chromatographic conditions were selected based on a thorough literature survey particularly relating to the physicochemical properties of the piperine such as its pKa, solubility, acidic nature, etc. most of the previously published studies have used organic solvents such as methanol, acetonitrile, etc. along with aqueous phase adjusted to high acidic pH and buffer solutions [21,22,23,24,25,26,27]. Further utilization of different column conditions, flow rate, and wavelength has also been observed. Moreover, the previously reported literature exhibits peaks with poor resolution or increased retention time. Taking a gist from the previous studies several preliminary trials were conducted, amongst them a mobile phase consisting of a systemic composition of Acetonitrile, water, and acetic acid as the pH modifier with suitable flow rate and wavelength was used for the quantitative estimation of piperine.

QbD based RP-HPLC method development

The predetermined quality characteristics that are known to enhance the method performance are referred to as Analytical target profile. The selection of ATP is purely dependent on the quality attributes that we want in the method. Defining of ATP is the primary step of AQbD concept. TheATP of the proposed analytical method is to achieve a good separation for quantification of Piperine, with lesser tailing factor and peak width along with acceptable analysis time. Based on the above-mentioned ATP, CQAs were identified as Tailing factor (Not more than 2) and Peak width (Not more than 2).

Method optimization by DoE

As per the adopted per 22 full factorial design, two independent variables, i.e., % concentration of Acetic acid in aqueous phase (X1) and mobile phase ratio (X2) were varied at two different levels that were coded for low and high (− 1 and + 1 respectively). Tailing factor (R1) and peak width (R2) were selected as the dependent or response variables. The DoE software was used to gain information on the critical values required to achieve the desired response of the selected independent variables.

The response, tailing factor (R1) and peak width (R2) obtained for each chromatographic trial are summarized in Table 1. Further, statistical optimization of the analytical method was performed by comparison of several statistical parameters, provided by Design-Expert® Software, Version 12. The statistical data of the applied design is summarized in Table 2. The relationship between the selected independent and dependent variables was derived by studying the mathematical expression in the form of polynomial equations. A positive sign of coefficient indicates a synergistic effect while a negative term indicates an antagonistic effect upon the response. The larger coefficient means that the independent variable has a more potent influence on the response. Graphical demonstration in the form of Response Surface Plot was generated (Fig. 2) to understand the effect of each factor on responses. The response surface plots provide an overview of the relationship between each dependent variable (CQA’s) and independent variables. Response Surface Plot shows the colored regions from blue to red indicating the intensity of the responses from lower to higher end. From the plot, it is observed that by decreasing the level of variable X1, i.e., concentration of acetic acid in the aqueous phase, the value of both the responses R1and R2 decreases which means that by decreasing the concentration of acetic acid in the aqueous phase tailing factor and peak width decreases. This further indicates that variable X1 has a significant impact on response R1 and R2 when compared to variable X2.

Table 1 Selected factor combinations for Piperine as per 22 full factorial design
Table 2 Summary of statistical parameters and polynomial equation
Fig. 2
figure 2

Response surface plot for optimization of HPLC method for Piperine

Establishment of MODR

Method operable design region (MODR) is a multidimensional combination and interaction of independent factors which further lead to the selection of acceptable operating ranges that assure quality. Figure 3 shows MODR (overlay plot) with the optimum region as a design space in yellow shade and selected method conditions were represented using flag. From the method operable Design region, analytical trial T1 (Conc. Of Acetic acid 0.05% and Mobile phase ratio 60:40) and T2 (Conc. Of Acetic acid 0.05% and Mobile phase ratio 70:30) falls under the region of successful operating ranges and fulfills the criteria of ATP and CQA for HPLC method. Among both, the trials T2 having Conc. Of Acetic acid 0.05% and Mobile phase ratio 70:30 was selected as optimized HPLC method due to its ability to give lesser tailing factor and peak width (Table 3).

Fig. 3
figure 3

MODR for optimization of HPLC method for Piperine

Table 3 Optimized chromatographic conditions

Quantitative estimation of Piperine

The optimized method was further used for the quantification of Piperine in crude P. nigrum fruits as well as in the extract. The quantitative estimation revealed the presence of 3.6%w/w and 5.62%w/w of Piperine in crude powder and extract respectively. The HPLC chromatograms for standard Piperine and samples have been depicted Fig. 4.

Fig. 4
figure 4

HPLC chromatograms for standard Piperine, crude P. nigrum L. fruits and extract

Method validation

The developed RP-HPLC method was validated to confirm its suitability for its intended purpose as described in ICH Q2 (R1) guidelines. The validation parameters of the proposed RP-HPLC method are summarized in Table 4 which was found to be within the standard limits specified in ICH Guidelines.

Table 4 Summary of validation parameters

The system suitability of the developed method was confirmed by the percent Relative Standard Deviation (RSD) of different parameters such as peak area, retention time (Rt), and tailing factor. The percent RSD of peak area, retention time, and tailing factor (< 2) were within the acceptable limits. The linear calibration curve for piperine was obtained for the selected concentration range (Fig. 5). The LOD and LOQ were determined from the linear regression data obtained from the calibration curve. The reproducibility and repeatability indicate the Precision of an analytical method. The lower intra-day and inter-day % RSD values for piperine demonstrated the high precision of the developed method. The % recovery of the piperine obtained from the sample indicates a good accuracy of the developed method.

Fig. 5
figure 5

Linear calibration curve for Piperine


The present research work was carried out to endeavor the development of Analytical Quality by design-assisted RP-HPLC method for estimation of Ppiperine in P.nigrum fruits. Though many studies have been conducted on the RP-HPLC method for analysis of Piperine, our study stands out amongst them as it reports the utilization of the Analytical QbD concept. With the help of AQbD principles, a suitable ATP has been developed which serves as a quality specification guide for the development of the analytical method. Based on the ATP, Critical Quality Attributes were identified. By taking into consideration the critical analytical parameters such as concentration of acids used in mobile phase and mobile phase ratios experiments were designed by DoE tools. Here, the CQAs were thoroughly examined by performing statistical analyses such as ANOVA. Polynomial equations and 3-D response surface plots were also developed for identifying the relationship between analytical parameters and CQAs. Further, the optimized chromatographic conditions were predicted from the MODR exhibiting yellow shaded region of successful operating ranges. The optimized chromatographic condition was then applied for quantitative estimation of Piperine in crude and extracted P. nigrum fruits which were found to be 3.6%w/w and 5.62%w/w, respectively. Apart from the development of AQbD assisted RP-HPLC method our study also reports the data on the Quality assessment of P. nigrum fruits with special reference to the estimation of toxic substances found in herbal crude drugs. The study has also represented the Phytochemical and Physico-chemical characteristics of the P. nigrum fruits. Valuable data on the determination of Aflatoxins, pesticide residues, and heavy metals have been reported which is mainly considered a prerequisite by regulatory authorities for ensuring the quality and safety of herbal drugs.


In the present research work, AnalyticalQbD-assisted RP-HPLC method was developed and validated for the quantitative estimation of Piperine in P. nigrum fruits and its extract. ATP and CQAs for the proposed method was outlined with the execution of chromatographic trials as per 22 full factorial design. Based on the obtained Design space, optimized chromatographic conditions were predicted. Furthermore, the crude P. nigrum was also extensively evaluated for quality control parameters, which was useful for ascertaining the quality of the herbal drug under study. Employment of the QbD tools has helped in developing a chromatographic method that has the potential of providing reproducible and reliable results along with the reduction in analysis time and cost. From the above findings, we can affirm that the application of the QbD approach for the standardization of herbal drugs can serve as an important tool for quality standardization of herbal drugs.

Availability of data and material

The data used to support the findings of this study are available from the corresponding author upon request.



Quality by Design


Reverse Phase High Performance Liquid Chromatography


Analytical Target Profile


Critical Quality Attributes


Design of Experiments


Method Operable Design Region


Limit of Detection


Limit of Quantification


  1. ParabGaonkar V, Hullatti K (2020) Indian traditional medicinal plants as a source of potent anti-diabetic agents: a review. J Diabetes Metab Disord 19(2):1895–1908

    Article  Google Scholar 

  2. Shi Z, Song D, Li R, Yang H, Qi L, Xin G, Wang D, Song H, Chen J, Hao H, Li P (2014) Identification of effective combinatorial markers for quality standardization of herbal medicine. J Chromatogr A 1345:78–85

    Article  CAS  Google Scholar 

  3. Gaonkar VP, Hullatti KK, Mannur V (2020) Standardization of Trigonella foenum-graecum L. seeds: a quality by design approach. Indian J Pharm Educ Res 54(4):1072–1079

    Article  Google Scholar 

  4. Liang Y, Xie P, Chan K (2004) Quality control of herbal medicine. J Chromatogr B 812:53–70

    Article  CAS  Google Scholar 

  5. Orlandini S, Pinzauti S, Furlanetto S (2012) Application of quality by design to the development of analytical separation methods. Anal Bioanal Chem 405:443–450

    Article  Google Scholar 

  6. ICH (2009) ICH Q8 (R2), Harmonised tripartite guideline, Pharmaceutical development.

  7. Yan B, Li Y, Guo Z, Qu H (2013) Quality by design for herbal drugs: a feedforward control strategy and an approach to define the acceptable ranges of critical quality attributes. Phytochem Anal 25:59–65

    Article  Google Scholar 

  8. Zhang L, Yan B, Gong X, Yu L, Qu H (2013) Application of quality by design to the process development of botanical drug products: a case study. AAPS PharmSciTech 14:277–286

    Article  CAS  Google Scholar 

  9. Parab Gaonkar V, Mannur VS, Mastiholimath VS, Hullatti K (2020) Development and evaluation of herbal supplement: a quality by design approach. Indian J Pharm Sci 82(4):640–649

    Google Scholar 

  10. Gurrala S, Shivaraj SCVS, Panikumar DA, Saraf G (2019) Analytical quality by design assisted hplc method for quantification of canagliflozin/metformin and stability studies. Indian J Pharm Educ Res 53:s699–s708

    Article  CAS  Google Scholar 

  11. Parab Gaonkar V, Hullatti K (2020) Quality assessment and RP-HPLC method development for estimation of curcuminoids in Curcuma longa: a quality by design approach. J Liq Chromatogr Relat Technol 44(1–2):95–102

    Google Scholar 

  12. Takooree H, Aumeeruddy MZ, Rengasamy KR, Venugopala KN, Jeewon R, Zengin G, Mahomoodally M (2019) A systematic review on black pepper (Piper nigrum L.): from folk uses to pharmacological applications. Crit Rev Food Sci Nutr 59:S210–S243

    Article  CAS  Google Scholar 

  13. Khare CP (2008) Indian medicinal plants an illustrated dictionary. Springer, Berlin

    Google Scholar 

  14. Sebastian P (2006) Ayurvedic medicine: the principles of traditional practice. Churchill Livingstone Elsevier, New York

    Google Scholar 

  15. World Health Organization (1998) Quality control methods for medicinal plant materials. WHO, Geneva

    Google Scholar 

  16. Khandelwal KR, Sethi V (2014) Practical pharmacognosy, 24th edn. Nirali Prakashan Maharashtra, Pune

    Google Scholar 

  17. World Health Organization (2007) WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. WHO, Geneva

    Google Scholar 

  18. Sylvester B, Tefas L, Vlase L, Tomuta I, Porfire A (2018) A quality by design (QbD) approach to the development of a gradient high-performance liquid chromatography for the simultaneous assay of curcuminoids and doxorubicin from long-circulating liposomes. J Pharm Biomed Anal.

    Article  PubMed  Google Scholar 

  19. ICH (1994) ICH Q2 (R1), harmonised tripartite guideline, validation of analytical procedures: text and methodology.

  20. Tripathy V, Basak BB, Varghese TS, Saha A (2015) Residues and contaminants in medicinal herb: a review. Phytochem Lett 14:67–78

    Article  CAS  Google Scholar 

  21. Khismatrao A, Bhairy S, Hirlekar S (2018) Development and validation of RP-HPLC method for simultaneous estimation of curcumin and piperine. Int J App Pharm 10(5):43–48

    Article  CAS  Google Scholar 

  22. Upadhyay V, Sharma N, Joshi HM, Malik A, Mishra M, Singh BP, Tripathi S (2013) Development and validation of rapid RP-HPLC method for estimation of piperine in Piper nigrum L. Int J Herb Med 1(4):6–9

    Google Scholar 

  23. Shah U, Patel S, Raval M (2018) Stability indicating reverse phase HPLC method for estimation of rifampicin and piperine in pharmaceutical dosage form. Curr Drug Discov Technol 15:54–64

    Article  CAS  Google Scholar 

  24. Rodriguez EL, Zhang C, Woolfork AG, Li Z, Bi C, Kaur H, Juritsch A, Moreau R, Hage D (2021) Analysis of curcumin and piperine in biological samples by reversed-phase liquid chromatography with multi-wavelength detection. J Chromatogr B 1162:122487

    Article  CAS  Google Scholar 

  25. Setyaningsih D, Santoso YA, Hartini YS, Murti YB, Hinrichs W, Patramurti C (2021) A Isocratic high-performance liquid chromatography (HPLC) for simultaneous quantification of curcumin and piperine in a microparticle formulation containing Curcuma longa and Piper nigrum. Heliyon 7:e06541

    Article  Google Scholar 

  26. Chaudhari VS, Borkarb RM, Murty US, Banerjee S (2020) Analytical method development and validation of reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous quantifications of quercetin and piperine in dual-drug loaded nano structured lipid carriers. J Pharm Biomed Anal 186:113325

    Article  CAS  Google Scholar 

  27. Tandel L, Sonone R, Jain V (2020) A novel reverse-phase high-performance liquid chromatographic method for simultaneous estimation of aloe-emodin and piperine in ayurvedic formulation. Asian J Pharm Clin Res 13(2):51–54

    CAS  Google Scholar 

Download references


The authors would like to sincerely thank the Principal, KLE College of Pharmacy, Belagavi for providing necessary facilities. Authors are also thankful to The Himalaya Drug Company, Bengaluru for providing standard Piperine as a gift sample.


Not applicable.

Author information

Authors and Affiliations



VPG carried out the experimental work. VSM and VPG equally contributed in framing and writing of manuscript. KKH revised and edited the final manuscript file. All authors have read and approved the manuscript.

Corresponding authors

Correspondence to Vishakha Parab Gaonkar or Vinodh Kumar Mannur.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Parab Gaonkar, V., Mannur, V.K. & Hullatti, K. Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L.. Futur J Pharm Sci 8, 16 (2022).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: